Kinetic Versus Endpoint Measurement for Quantitative Histochemical Determination of Enzyme Activity in Muscle Fibers
نویسندگان
چکیده
منابع مشابه
application and construction of carbon paste modified electrodes developed for determination of metal ions in some real samples
ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...
15 صفحه اولEnzyme patterns in single human muscle fibers.
Individual muscle fibers from the biceps of five individuals were analyzed for 10 different enzymes. For each person, lactic dehydrogenase and adenylokinase were first measured in 50 to 100 fibers. From each large group, 20 or more fibers, representing the full range of enzyme activity, were analyzed in duplicate for the other eight enzymes (glycogen phosphorylase, P-fructokinase, pyruvate kina...
متن کاملHistochemical Studies on the Red, White and Intermediate Muscle Fibers of Some Skeletal Muscles Iii. Histochemical Demonstration of Oxidative Enzymes, Phosphorylase and Glycogen in Respiratory Muscle Fibers
1) Three different types of muscle fibers were clearly distinguished in the intercostal muscles and the diaphragm of human, cat and rat by histochemical demonstration of oxidative enzymes, phosphorylase and glycogen. 2) The intercostal muscles and diaphragm each presented dissimilar patterns of the muscle fibers. The diaphragm did not show any definite correlation between the diameter and the h...
متن کاملQuantitative determination of phenoxymethylpenicillin using kinetic method
The kinetic of conjugated reactions of S-oxidation and perhydrolysis of phenoxymethylpenicillin with potassium hydrogenperoxomonosulfate in alkaline medium has been studied by the light absorbance increase of a forming product at 269 nm. The procedure of the quantitative analysis of phenoxymethylpenicillin in powder and drug by the kinetic spectrophotometric method is elaborated using triple po...
متن کاملHistochemical studies on the red, white and intermediate muscle fibers of some skeletal muscles. I. Succinic dehydrogenase activity and physiological function of intercostal muscle fibers.
From the histochemical study of the intercostal muscles of cat, the following results were obtained. 1. Three different types of muscle fibers have been clearly distinguished in intercostal muscles by histochemical demonstration of succinic dehydrogenase; namely, the white fibers are stained faintly, while the red fibers deep blue and the intermediate fibers purple or bluish purple. 2. The diff...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1998
ISSN: 0022-1554,1551-5044
DOI: 10.1177/002215549804600217